RBS

Part:BBa_K4634001:Design

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-09-25)


pET28a(+) backbone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5242
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5242
    Illegal BglII site found at 5130
    Illegal XhoI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5242
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5242
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5257
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 303
    Illegal NgoMIV site found at 3350
    Illegal NgoMIV site found at 3510
    Illegal NgoMIV site found at 5098
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2429


Design Notes

NA


Source

Get from Snapgene "Plasmid Files" website: https://www.snapgene.com/plasmids/pet_and_duet_vectors_(novagen)/pET-28a(%2B)

References